Given that the 16S profiles through the gut appear really various for intracellular and extracellular DNA [33], qPCR is predicted to replicate the NGS-targeted community structure both of those quantitatively and qualitatively a lot more intently than flow cytometry.
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Precisely the same DNA extract serves as the starting product both equally for qPCR and NGS, making qPCR finished in ninety six- or 384-format easy to carry out during the workflow for prime-throughput Evaluation of around thousands of microbiome samples.
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